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Assisted selection using molecular markers linked to rust resistance SH3 gene in Coffea arabica

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dc.contributor.author Silva, Angelita Garbossi
dc.contributor.author Ariyoshi, Caroline
dc.contributor.author Shigueoka, Luciana Harumi
dc.contributor.author Pereira, Luiz Filipe Protasio
dc.contributor.author Sera, Gustavo Hiroshi
dc.date.accessioned 2024-05-16T00:06:21Z
dc.date.available 2024-05-16T00:06:21Z
dc.date.issued 2023-10-25
dc.identifier.citation SILVA, A. G. et al. Assisted selection using molecular markers linked to rust resistance SH3 gene in Coffea arabica. Crop Breeding and Applied Biotechnology, Viçosa, v. 23, n. 4, p. 1-6, 25 oct. 2023. pt_BR
dc.identifier.uri http://dx.doi.org/10.1590/1984-70332023v23n4a48 pt_BR
dc.identifier.uri http://www.sbicafe.ufv.br/handle/123456789/14360
dc.description.abstract The aim of this work was to validate markers linked to the SH3 gene of coffee leaf rust (CLR) resistance and use them in assisted selection. Initially, we validated the markers in genotypes already known to carry SH3. Next, we performed phenotype and genotype evaluation for resistance to CLR in coffee plants growing under field conditions. We used Arabica coffee progenies derived from BA-10, which carries the SH3 gene due to introgression of C. liberica. Three SCAR markers (SP-M16-SH3, BA-48-21O-f, and BA-124-12K-f) and one SSR marker (Sat244) linked to SH3 gene were used to amplify the coffee plants’ DNA. Our assessments of markers validation in resistant genotypes, SP-M16-SH3 and BA-124-12K-f, were efficient to identify the SH3 gene. These two markers were used to evaluate the progenies derived from BA-10 and were significantly linked to the phenotype evaluations. The SP-M16-SH3 marker was more efficient, with the advantage of being codominant. pt_BR
dc.format pdf pt_BR
dc.language.iso en pt_BR
dc.publisher Crop Breeding and Applied Biotechnology pt_BR
dc.rights Open Access pt_BR
dc.subject BA-10 genotype pt_BR
dc.subject Coffee breeding pt_BR
dc.subject Hemileia vastatrix pt_BR
dc.subject Markers validation pt_BR
dc.subject SCAR markers pt_BR
dc.subject.classification Cafeicultura::Genética e melhoramento pt_BR
dc.title Assisted selection using molecular markers linked to rust resistance SH3 gene in Coffea arabica pt_BR
dc.type Artigo pt_BR

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