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Comparison of high performance liquid chromatography with fluorescence detector and with tandem mass spectrometry methods for detection and quantification of ochratoxin a in green and roasted coffee beans

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dc.contributor.author Bandeira, Raquel Duarte da Costa Cunha
dc.contributor.author Uekane, Thais Matsue
dc.contributor.author Cunha, Carolina Passos da
dc.contributor.author Rodrigues, Janaina Marques
dc.contributor.author Cruz, Marcus Henrique Campino de la
dc.contributor.author Godoy, Ronoel Luiz de Oliveira
dc.contributor.author Fioravante, Andreia de Lima
dc.date.accessioned 2019-11-08T12:44:24Z
dc.date.available 2019-11-08T12:44:24Z
dc.date.issued 2013-11
dc.identifier.citation BANDEIRA, R. D. C. C. et al. Comparison of high performance liquid chromatography with fluorescence detector and with tandem mass spectrometry methods for detection and quantification of ochratoxin a in green and roasted coffee beans. Brazilian Archives of Biology and Technology, Curitiba, v. 56, n. 6, p. 911-920, nov./dez. 2013. pt_BR
dc.identifier.issn 1678-4324
dc.identifier.uri http://dx.doi.org/10.1590/S1516-89132013000600004 pt_BR
dc.identifier.uri http://www.sbicafe.ufv.br/handle/123456789/12378
dc.description.abstract Two analytical methods for the determination and confirmation of ochratoxin A (OTA) in green and roasted coffee samples were compared. Sample extraction and clean-up were based on liquid–liquid phase extraction and immunoaffinity column. The detection of OTA was carried out with the high performance liquid chromatography (HPLC) combined either with fluorescence detection (FLD), or positive electrospray ionization (ESI+) coupled with tandem mass spectrometry (MS–MS). The results obtained with the LC-ESI-MS/MS were specific and more sensitive, with the advantages in terms of unambiguous analyte identification, when compared with the HPLC-FLD. pt_BR
dc.format pdf pt_BR
dc.language.iso en pt_BR
dc.publisher Instituto de Tecnologia do Paraná - Tecpar pt_BR
dc.relation.ispartofseries Brazilian Archives of Biology and Technology;v.56, n.6, p.911-920, 2013;
dc.rights Open Access pt_BR
dc.subject HPLC-FLD pt_BR
dc.subject LC-MS/MS pt_BR
dc.subject Green Coffee pt_BR
dc.subject Roasted Coffee pt_BR
dc.subject.classification Cafeicultura::Qualidade de bebida pt_BR
dc.title Comparison of high performance liquid chromatography with fluorescence detector and with tandem mass spectrometry methods for detection and quantification of ochratoxin a in green and roasted coffee beans pt_BR
dc.type Artigo pt_BR

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