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Isolation and identification of a native microbial consortium for the coffee pulp degradation above 2000 masl

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dc.contributor.author Cuba, Marcela N. Arteaga
dc.contributor.author Dilas-Jiménez, Josué O.
dc.contributor.author Díaz, Norberto Villanueva
dc.contributor.author Miranda, Ofelia C. Cernaqué
dc.contributor.author Santillán, Segundo M. Tafur
dc.contributor.author García, José Enrique Olivera
dc.contributor.author Mugruza-Vassallo, Carlos A.
dc.date.accessioned 2021-09-15T10:15:42Z
dc.date.available 2021-09-15T10:15:42Z
dc.date.issued 2021
dc.identifier.citation CUBA, M. N. A. et al. Isolation and identification of a native microbial consortium for the coffee pulp degradation above 2000 masl. Coffee Science, Lavras, v. 16, p. 1-13, 2021. pt_BR
dc.identifier.issn 1984-3909
dc.identifier.uri https://doi.org/10.25186/.v16i.1810 pt_BR
dc.identifier.uri http://www.sbicafe.ufv.br/handle/123456789/12796
dc.description.abstract Pulp and mucilage constitute the most abundant by-products of the processing of coffee and inadequate handling produces high-rates of pollution. Considering that in nature there is a large variety of cellulolytic microorganisms which can intervene in the degradation of coffee pulp, the inclusion of a native microbial cellulolytic consortium was assessed. The sample for isolation consisted of coffee pulp and soil from a humid forest at more than 2,000 masl. Nutrient agar was used as a means of isolation for bacteria, and malt agar for fungi, supplemented with 50% soil extract and 1% carboxylmethylbcellulose. This achieved the isolation of 118 strains of bacteria and 114 of fungi. Cellulolytic activity was established using the filter paper test, assessing and selecting only those that presented higher glucose production, among them 12 strains of fungi and 11 strains of bacteria. To obtain the microbial consortium, randomized blends were performed for both fungi and bacteria, again assessing the production of glucose. The bacterial consortium was made up of Ochrobactrum pseudo grignonense, Paenibacillus lauruscon and Bacillus xiamenensis and the fungal consortium by Fusarium sp., Penicillum sp., Cylindrocarpon sp. The optimal treatment achieved a complete degradation of the pulp in 28 days, that would contribute to the recovery and conservation of the coffee ecosystem. The main interpretation was that a wet environment at more than 2,000 masl is still suitable for coffee composting but different bacterial and fungi consortia were found to support other recent work done with one type of consortium. pt_BR
dc.format pdf pt_BR
dc.language.iso en pt_BR
dc.publisher Editora UFLA pt_BR
dc.relation.ispartofseries Coffee Science:v.16;
dc.rights Open Access pt_BR
dc.subject Carboxymethylcellulose (CMC) pt_BR
dc.subject Cellulose pt_BR
dc.subject Coffee pulp pt_BR
dc.subject Degradation pt_BR
dc.subject Microbial consortium pt_BR
dc.subject Meters above sea level (masl) pt_BR
dc.subject.classification Cafeicultura::Colheita, pós-colheita e armazenamento pt_BR
dc.title Isolation and identification of a native microbial consortium for the coffee pulp degradation above 2000 masl pt_BR
dc.type Artigo pt_BR

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