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Exploratory studies for cryopreservation of Coffea arabica L. seeds

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dc.contributor.author Figueiredo, Madeleine Alves de
dc.contributor.author Coelho, Stefania Vilas Boas
dc.contributor.author Rosa, Sttela Dellyzete Veiga Franco da
dc.contributor.author Vilela, Amanda Lima
dc.contributor.author Silva, Luciano Coutinho
dc.date.accessioned 2022-02-08T13:37:41Z
dc.date.available 2022-02-08T13:37:41Z
dc.date.issued 2017
dc.identifier.citation FIGUEIREDO, M. A. et al. Exploratory studies for cryopreservation of Coffea arabica L. seeds. Journal of Seed Science, Londrina, v. 39, n. 2, p. 150-158, abr./jun. 2017. pt_BR
dc.identifier.issn 2317-1545
dc.identifier.uri http://dx.doi.org/10.1590/2317-1545v39n2171123 pt_BR
dc.identifier.uri http://www.sbicafe.ufv.br/handle/123456789/13293
dc.description.abstract Cryopreservation is a viable option for conservation of coffee germplasm. However, for this technique to be completely successful, it is of fundamental importance to carry out studies that ensure maintenance of cell integrity before and after immersion in liquid nitrogen (LN). Therefore, the aim in this study was to investigate the water content, cooling rate, and final temperature most suitable for cryopreservation of Coffea arabica L. seeds. The seeds were dried by silica gel to water contents of 5, 10, 15, 20, 30 and 40 % wb, subjected to slow cooling treatments at speeds of -1, -3 and -5 °C min.-1 to final temperatures of -40, -50 and -60 °C and then directly immersed in LN. After storage, seeds were rewarmed at 40 °C for two minutes. The survival rate and viability of the seeds and embryos were evaluated by the tetrazolium and germination tests. Results of the tetrazolium test indicate that embryos excised from cryopreserved seeds are less sensitive to cryopreservation than whole seeds are. The water content of 20% wb and the use of zygotic embryos led to the highest survival rate of the coffee seeds, depending on the cooling rate and the final temperature of precooling. pt_BR
dc.format pdf pt_BR
dc.language.iso en pt_BR
dc.publisher Associação Brasileira de Tecnologia de Sementes - ABRATES pt_BR
dc.relation.ispartofseries Journal of Seed Science;v.39, n.2, 2017
dc.rights Open Access pt_BR
dc.subject Sílica gel pt_BR
dc.subject Teor de água pt_BR
dc.subject Resfriamento lento pt_BR
dc.subject Nitrogênio líquido pt_BR
dc.subject.classification Cafeicultura::Sementes e mudas pt_BR
dc.title Exploratory studies for cryopreservation of Coffea arabica L. seeds pt_BR
dc.type Artigo pt_BR

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