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Comparison of extraction methods for kahweol and cafestol analysis in roasted coffee

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dc.contributor.author Dias, Rafael C. E.
dc.contributor.author Faria, Adelia F. de
dc.contributor.author Mercadante, Adriana Z.
dc.contributor.author Bragagnolo, Neura
dc.contributor.author Benassi, Marta de T.
dc.date.accessioned 2022-04-02T13:43:58Z
dc.date.available 2022-04-02T13:43:58Z
dc.date.issued 2013
dc.identifier.citation DIAS, R. C. E. et al. Comparison of extraction methods for kahweol and cafestol analysis in roasted coffee. Journal of the Brazilian Chemical Society, São Paulo, v. 24, n. 3, p. 492-499, mar. 2013. pt_BR
dc.identifier.issn 1678-4790
dc.identifier.uri https://doi.org/10.5935/0103-5053.20130057 pt_BR
dc.identifier.uri http://www.sbicafe.ufv.br/handle/123456789/13423
dc.description.abstract Kahweol and cafestol, diterpenes from the unsaponifiable fraction of coffee, present known effects on human health such as anticarcinogenic and hipercholesterolemic activities. There are discrepancies regarding the levels reported for these compounds in roasted coffee, probably due to the extraction processes. Therefore, four sample preparation methods were studied: direct hot saponification (DHS), direct cold saponification (DCS); and Bligh and Dyer (BD) or Soxhlet (SO) extraction followed by saponification. The levels of diterpenes and their dehydro derivatives obtained by high performance liquid chromatography with diode array and mass spectrometry detectors (HPLC-DAD-MS/MS) and the chromatographic profiles of roasted coffee, obtained by these four methods, were compared. DHS was more efficient for extraction, showing better separation of chromatographic peaks and levels of 930.2 (± 36.8), 113.2 (± 4.7), 568.6 (± 16.6) and 87.1 (± 3.7) mg 100 g–1 for kahweol, dehydrokahweol, cafestol and dehydrocafestol, respectively. The DHS extract presented a diterpene content (kahweol and cafestol) 15% superior to that of DCS and up to 88% superior than using SO and BD methods. pt_BR
dc.format pdf pt_BR
dc.language.iso en pt_BR
dc.publisher Sociedade Brasileira de Química pt_BR
dc.relation.ispartofseries Journal of the Brazilian Chemical Society;v.24, n.3, 2013
dc.rights Open Access pt_BR
dc.subject Direct saponification pt_BR
dc.subject Soxhlet pt_BR
dc.subject Bligh and Dyer pt_BR
dc.subject Diterpenes pt_BR
dc.subject Dehydroditerpenes pt_BR
dc.subject.classification Cafeicultura::Colheita, pós-colheita e armazenamento pt_BR
dc.title Comparison of extraction methods for kahweol and cafestol analysis in roasted coffee pt_BR
dc.type Artigo pt_BR

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