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The effect of steeping robusta coffee beans on monocytes:expression of IL-1β and TNF-α against streptococcus mutans

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dc.contributor.author Dewanti, I Dewa Ayu Ratna
dc.contributor.author Lestari, Pujiana Endah
dc.contributor.author Budirahardjo, Roedy
dc.contributor.author Setyorini, Dyah
dc.contributor.author Yani, RistyaWidi Endah
dc.contributor.author Wibisono, Sunlip
dc.contributor.author Mel, Maizirwan
dc.date.accessioned 2021-05-03T13:15:03Z
dc.date.available 2021-05-03T13:15:03Z
dc.date.issued 2019-10
dc.identifier.citation DEWANTI, I D. A. R. et al. The effect of steeping robusta coffee beans on monocytes:expression of IL-1β and TNF-α against streptococcus mutans. Coffee Science, Lavras, v. 14, n. 4, p. 477-483, out./dez. 2019. pt_BR
dc.identifier.issn 1984-3909
dc.identifier.uri http://dx.doi.org/10.25186/cs.v14i4 pt_BR
dc.identifier.uri http://www.sbicafe.ufv.br/handle/123456789/12734
dc.description.abstract Adhesion, IL–1β, TNF–α are components that affect in inflammation. So, the effect of steeping green and black Robusta coffee beans to adhesion of Streptococcus mutans on this components. This study used monocytes isolated from healthy human peripheral blood using Ficoll-Hypaque centrifugation method. Monocytes were divided into eight groups, i. e. (i) Control group (untreated monocytes), (ii) S. mutans group (monocytes + S. mutans), (iii) Black Coffee 2.5 % group (monocytes + black coffee beans 2.5 % + S. mutans), (iv) Black Coffee 5 % group (monocytes + black coffee beans 5 % + S. mutans), (v) black Coffee 10 % group (monocytes + black coffee beans 10 % + S. mutans), (vi) Green Coffee 2.5 % group (monocytes + green coffee beans 2.5 % + S. mutans), (vii) Green Coffee 5 % group (monocytes + green coffee beans 5 % + S. mutans), (viii) Green coffee 10 % group (monocytes + green coffee beans 10 % + S. mutans). S. mutans adhesion on monocytes was analyzed using histochemistry method, while immunocytochemical staining was used for analyzing IL–1β and TNF–α. Cells counting was done per 100 monocytes under a light microscope with 400 x magnification. Data were analyzed using ANOVA followed by LSD test. Results showed that steeping green and black Robusta coffee beans increased the adhesion of S. mutans on monocytes, but it decreased of IL–1β, TNF–α expression (P <0.05). In conclusion, steeping of Robusta coffee beans increased adhesion and decreased IL-1β, TNF-α against S. mutans. pt_BR
dc.format pdf pt_BR
dc.language.iso pt_BR pt_BR
dc.publisher Editora UFLA pt_BR
dc.relation.ispartofseries Coffee Science:v.14,n.4;
dc.rights Open Access pt_BR
dc.subject Black coffee pt_BR
dc.subject Cytokine pt_BR
dc.subject Green coffee pt_BR
dc.subject Immunocytochemical pt_BR
dc.subject Inflammation pt_BR
dc.subject.classification Cafeicultura::Café e saúde pt_BR
dc.title The effect of steeping robusta coffee beans on monocytes:expression of IL-1β and TNF-α against streptococcus mutans pt_BR
dc.type Artigo pt_BR

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